prlr  (Santa Cruz Biotechnology)

Journal: PLoS ONE

Article Title: MICA-G129R: A bifunctional fusion protein increases PRLR-positive breast cancer cell death in co-culture with natural killer cells

doi: 10.1371/journal.pone.0252662

Figure Lengend Snippet: The cytotoxicity of NK-92 cells on T-47D cells was measured at different conditions. A. T-47D cells were co-cultured with NK-92 cells at different effector/target ratios 5:1, 2:1, 1:1, and 1:2 for 24 hours with control or MICA-G129R conditioned media. B. The co-culture of T-47D cells and NK-92 cells at the ratio of 1:1 was treated with the control, MICA-G129R, MICA or G129R conditioned media for 24 hours. C. The NK-92 cells were co-cultured with T-47D cells, HeLa cells (PRLR-negative), 293 (PRLR-negative) or PRLR ectopic expressed 293 cells at the ratio of 1:1 with control or MICA-G129R conditioned media for 24 hours. Data are presented as mean ± SD (n = 3). * indicates P<0.05; ** indicates P<0.01.

Article Snippet: The following antibodies were used in Western blot: monoclonal mouse anti-human MICA (sc-137242), PRL (sc-46698), PRLR (sc-377098) antibodies, mouse IgG light chain binding protein conjugated to HRP (mIgG BP-HRP) (sc-516102) as secondary antibody (purchased from Santa Cruz Biotechnology, CA, USA) and monoclonal mouse anti-V5 antibody (#80076) (purchased from Cell Signaling Technology, MA, USA).

Techniques: Cell Culture, Control, Co-Culture Assay